==> jsc. <== Test Data

(12f6.2)

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Logit-Y Vs Log of concentration program for computation of Harmon concentration in RIA

RadioImmunoAssay Principle and Practice MRA Pillai and SD Bhandarkar, BARC, Bombay (1994)

Computer Program by

A.S. Ghai

Technical Officer

Computer Centre, N.D.R.I., Karnal - 132 001

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Non Specific Binding(NSB) = 116.5000

Maximum Binding(MB) = 2867.8899

Std. Counts ......

2700.0400 2312.2800 2107.1399 1593.4600 1430.1700 1235.2100 897.7700 504.2200 429.7000 287.7900

Concentration.....

9.0000 19.0000 39.0000 78.0000 156.0000 312.5000 625.0000 1250.0000 2500.0000 5000.0000

1 0.95 2.73

2 1.28 1.37

3 1.59 0.96

4 1.89 0.15

5 2.19 -0.09

6 2.49 -0.38

7 2.80 -0.92

8 3.10 -1.81

9 3.40 -2.05

10 3.70 -2.71

NO OF OBSERVATIONS= 10

MEANS

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2.3393831 -0.27478245

STD.DEV.

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0.91896117 1.6798223

2 1

VARIANCE COVARIANCE MATRIX

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25.396227 -13.727107

-13.727107 7.6004066

REG. COFFICIENT

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-1.8061016

SE OF REG COEFFICIENTS

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0.99641256E-01

PURE CONSTANT INTERCEPT = 3.9503810

T VALUE FOR EACH COEFFICIENT

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-18.126041

CORRELATION MATRIX

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1 2

1.0000

-0.9880 1.0000

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Sr.No. Counts % Binding Logit-Y Log of Conc. CONCENTRAION Mean of 2 Conc.

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1 1814.0900 61.6994 0.4768 1.9232 83.8000

2 1861.2300 63.4127 0.5500 1.8827 76.3378 80.0689

3 2058.3401 70.5767 0.8749 1.7028 50.4454

4 2066.0200 70.8558 0.8884 1.6954 49.5859 50.0156

5 2463.7100 85.3100 1.7591 1.2133 16.3400

6 2470.1799 85.5451 1.7780 1.2028 15.9512 16.1456

7 2596.4299 90.1337 2.2122 0.9624 9.1709

8 2940.1699 102.6270 0.0000 0.9624 0.0000 4.5854

9 2130.5701 73.2019 1.0049 1.6309 42.7420

10 2222.8799 76.5569 1.1835 1.5320 34.0397 38.3908

11 2437.2100 84.3468 1.6843 1.2547 17.9763

12 2468.2600 85.4753 1.7724 1.2059 16.0662 17.0213